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X-RAY CRYSTALLOGRAPHY & MOLECULAR CHARACTERIZATION FACILITY

Director, X-ray Crystallography: John Pascal, PhD Office: 804 BLSB Tel: (215) 503-4596
Director, Molecular Characterization: Michael Root, MD, PhD Office: 802 BLSB Tel: (215) 503-4564
Facility Manager: Anshul Bhardwaj, PhD Office: 822 or 826 BLSB Tel: (215) 503-4587
Location: 822 BLSB 233 S. 10th Street Philadelphia, PA 19107	Contact: Tel: (215) 503-4587 Email:Anshul.Bhardwaj@Jefferson.edu

This facility serves the needs of KCC research groups in crystallization, data collection, structure solution, refinement, structural interpretation and mutagenesis of macromolecules. The facility has a HydraPlusOne crystallization robot for high-throughput crystallization screening and maintains an Enhance Ultra sealed tube X-ray generator with Osmic Confocal Max-Flux optis, Xcalibur kappa 4-circle diffractometer, Cryojet XL and a 165 mm Onyx CCD detector. The facility has a molecular graphics area with the computational capabilities and the software for data collection, data reduction, structure determination and refinement.

The facility also provides access to instrumentation for biophysical characterization of proteins, and protein/protein and protein/DNA interactions. These instruments include a Beckman Coulter Analytical Ultracentrifuge (ProteomeLab XL-I Protein Characterization System), a JASCO J-810 Spectropolarimeter, a BIACORE 3000 SPR biosensor, a KinExA 3000, and an Isothermal Titration Calorimeter.

John M. Pascal, Ph.D.	Facility Director
Michael J. Root, Ph.D.	Facility Co-Director

X-ray Crystallography and Molecular Characterization Resource ReservationSystem